IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/18

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12:00 PM

started 5 mL culture of MG1655 for genomic prep. incubated in roller at 37°C

5:15 PM

Took OD reading of 5 mL culture, 1.708A. used 1.5 mL to pellet for genomic prep (qiagen: DNeasy kit). started overnight incubation in buffer ATL/proteinase K, 56°C at 5:50 PM.