IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/15

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7/15/10

8:45 AM

Inactivated yesterday's ligations

Transformations

100 μL cells, 2 μL Ligation product, electroporate, add 1 mL LB, recover for 1 hour at 37°C.

Samples: pSB1T3-pArsR, pSB1T3-RpoS pSB1T3-ArsR

Plated 200 μL at 10:35 AM, incubated at 37°C