IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/22

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6/22/10

re-running gel

lane sample amount
1. 1 Kb ladder 3 ul + .6 ul dye
2. Ges ABC 5 ul + 1 ul dye
3. GolT 5 ul + 1 ul dye
4. golB 5 ul + 1 ul dye
5. ArsR 5 ul + 1 ul dye
6. Chlor backbone 5 ul + 1 ul dye
7. Tet backbone 5 ul + 1 ul dye
8. Amp backbone 5 ul +1 ul dye
9. (+) control golS 5 ul + 1 ul dye
10. 100 bp ladder 5 ul + 1 ul dye

PCR

Ingredient Amount/Reaction Mastermix (9 reactions)
10X Buffer 4 ul 36 ul
10mM dNTPs 1 ul 9 ul
DNA 2.5 ul 22.5 ul
F Primer .4 ul added individually to each reaction
R primer .4 ul added individually to each reaction
Polymerase .4 ul 3.6 ul
H2O 31.3 ul 281.7 ul

Samples: ArsR, ArsB, PAL, negative control (water).

ArsR and PAL produced bands, no band in ArsB.