IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/22

UIUC - Arsenic Collection System!

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6/22/10 re-running gel lane sample amount 1. 1 Kb ladder 3 ul + .6 ul dye 2. Ges ABC 5 ul + 1 ul dye 3. GolT 5 ul + 1 ul dye 4. golB 5 ul + 1 ul dye 5. ArsR 5 ul + 1 ul dye 6. Chlor backbone 5 ul + 1 ul dye 7. Tet backbone 5 ul + 1 ul dye 8. Amp backbone 5 ul +1 ul dye 9. (+) control golS 5 ul + 1 ul dye 10. 100 bp ladder 5 ul + 1 ul dye PCR Ingredient Amount/Reaction Mastermix (9 reactions) 10X Buffer 4 ul 36 ul 10mM dNTPs 1 ul 9 ul DNA 2.5 ul 22.5 ul F Primer .4 ul added individually to each reaction R primer .4 ul added individually to each reaction Polymerase .4 ul 3.6 ul H2O 31.3 ul 281.7 ul Samples: ArsR, ArsB, PAL, negative control (water). ArsR and PAL produced bands, no band in ArsB.