IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/21

UIUC - Arsenic Collection System!

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6/21/10 PCR Ingredient Amount/Reaction Mastermix (9 reactions) 10X Buffer 4 ul 36 ul 10mM dNTPs 1 ul 9 ul DNA 2.5 ul 22.5 ul F Primer .4 ul added individually to each reaction R primer .4 ul added individually to each reaction Polymerase .4 ul 3.6 ul H2O 31.3 ul 281.7 ul 39.2 ul mastermix, .4 ul F primer, .4 ul R primer to each reaction. Primers at 100uM. Program: iGEM 2Kb Samples: Sample F Primer R Primer ArsR ArsR F RFC10 ArsR R RFC10 ArsB ArsB F RFC10 ArsB R RFC10 PAL PAL RFC10 F PAL RFC10 R (-) control H2O H2O