IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/02/12

iGEM Project name 1

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February 12th 2010 Wi-Fi coli: Experimental and Modeling design Objective Get an idea of final construction. Looking for: Assembly of all necessary bio-bricks (are they already assembled?) Looking for bio-bricks and methods Protocols (strain, reactants, medium) We need to prove if it is possible for a luciferase emitter to stimulate a chromophore receptor. Plasmid structure Vectors must have the same structure, this structure is an iGEM standard, all bio-bricks have the same standard unless specified. Standard assembling Bio-bricks must contain: Suffix and Prefix: These are sequences placed at both sides of the sequence to be inserted; these sequences contain several restriction sites in order to excise the bio-brick if necessary. It is important to notice that the principal sequence placed between the suffix and prefix sequences must lack restrictions sites contained in suffix/prefix sequences to avoid bio-brick damaging. Origin of replication Expressed sequence RBS (Ribosome binding site) Promoter Gene Terminus Resistance gene The sequence to be expressed should be like this: Suffix--rbs—promoter—ATG—prot—stop codon--prefix We need to search for bio-brick’s specifications like kind of restriction-sites, resistance gene, bio-brick’s length, etc. Modeling It is necessary to model: Signal input Transcriptional response after receptor stimulation depending on the number of photons received. How strong was the transcriptional response after a defined photon input. What do we expect of our model? Modeling signal input and transcriptional response. We need to model: elements, circuit, parameters, logic design.