IGEM:UNAM/2009/Notebook/IGEM project/2011/07/29

iGEM Project name 1

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Digestion Today Vlad and me selected the best extraction of plasmid to do the digestion: Selection of plasmid: Gel 8. Plasmid(27/jul/11) Line 1 -> J23110A Line 2 -> J23110B Line 3 -> J23110C Line 4 -> J23110D Line 6 -> J23111B Line 7 -> J23111C Line 10 -> J23112B Line 11 -> J23112C Line 13 -> J23113A Line 14 -> J23113B Gel 9. Plasmid (27/jul/11) Line 4 -> J23114C Line 9 -> J23115D Line 11 -> J23116B Line 12 -> J23116C Line 13 -> J23116D Gel 10. Plasmid (28/jul/11) Line 1 -> J23117C Line 3 -> J23118A Line 4 -> J23118B Line 5 -> J23118C Line 6 -> J23118D Line 7 -> J23119A Line 11 -> J23100A Line 12 -> J23100B Line 13 -> J23100C Line 14 -> J23100D Line 15 -> J23101A Gel 11. Plasmid (28/jul/11) Line 3 -> J23101B Line 4 -> J23101C Line 5 -> J23101D Line 6 -> J23102A Line 7 -> J23102B Line 9 -> J23102D Line 10 -> J23103A Line 11 -> J23103B Line 12 -> J23103C Line 13 -> J23103D Line 15 -> J23104B Gel 12. Plasmid (28/jul/11) Line 1 -> J23104C Line 2 -> J23104D Line 3 -> J23105A Line 4 -> J23105B Line 5 -> J23105C Line 6 -> J23105D Line 7 -> J23106A Line 8 -> J23106B Line 9 -> J23106C Line 10 -> J23106D Line 11 -> J23107A Line 12 -> J23107B Line 13 -> J23107C Line 14 -> J23107D Line 15 -> J23108A Reaction of the digestion: Digestion Reagent Quantity H2O miliq 6 μl Buffer 10X of EcoRI 1 μl plasmid 2 μl EcoRI enzyme 1 μl Total 10 μL.