IGEM:UNAM/2009/Notebook/IGEM project/2011/07/28

iGEM Project name 1

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Gel of plasmid Today we did four gels (agarose 1%) of the last plasmid´s extraction. Gel 10. Plasmid (15 lines) Line 1 -> 3 μl of plasmid J23117C + 1 μl of dye. Line 2 -> 3 μl of plasmid J23117D + 1 μl of dye. Line 3 -> 3 μl of plasmid J23118A + 1 μl of dye. Line 4 -> 3 μl of plasmid J23118B + 1 μl of dye. Line 5 -> 3 μl of plasmid J23118C + 1 μl of dye. Line 6 -> 3 μl of plasmid J23118D + 1 μl of dye. Line 7 -> 3 μl of plasmid J23119A + 1 μl of dye. Line 8 -> 3 μl of plasmid J23119B + 1 μl of dye. Line 9 -> 3 μl of plasmid J23119C + 1 μl of dye. Line 10 -> 3 μl of plasmid J23119D + 1 μl of dye. Line 11 -> 3 μl of plasmid J23100A + 1 μl of dye. Line 12 -> 3 μl of plasmid J23100B + 1 μl of dye. Line 13 -> 3 μl of plasmid J23100C + 1 μl of dye. Line 14 -> 3 μl of plasmid J23100D + 1 μl of dye. Line 15 -> 3 μl of plasmid J23101A + 1 μl of dye. Gel 11. Plasmid (15 lines) Line 1 -> Empty. Line 2 -> Empty. Line 3 -> 3 μl of plasmid J23101B + 1 μl of dye. Line 4 -> 3 μl of plasmid J23101C + 1 μl of dye. Line 5 -> 3 μl of plasmid J23101D + 1 μl of dye. Line 6 -> 3 μl of plasmid J23102A + 1 μl of dye. Line 7 -> 3 μl of plasmid J23102B + 1 μl of dye. Line 8 -> 3 μl of plasmid J23102C + 1 μl of dye. Line 9 -> 3 μl of plasmid J23102A + 1 μl of dye. Line 10 -> 3 μl of plasmid J23103A + 1 μl of dye. Line 11 -> 3 μl of plasmid J23103B + 1 μl of dye. Line 12 -> 3 μl of plasmid J23103C + 1 μl of dye. Line 13 -> 3 μl of plasmid J23103D + 1 μl of dye. Line 14 -> 3 μl of plasmid J23104A + 1 μl of dye. Line 15 -> 3 μl of plasmid J23104B + 1 μl of dye. Gel 12. Plasmid (15 lines) Line 1 -> 3 μl of plasmid J23104C + 1 μl of dye. Line 2 -> 3 μl of plasmid J23104D + 1 μl of dye. Line 3 -> 3 μl of plasmid J23105A + 1 μl of dye. Line 4 -> 3 μl of plasmid J23105B + 1 μl of dye. Line 5 -> 3 μl of plasmid J23105C + 1 μl of dye. Line 6 -> 3 μl of plasmid J23105D + 1 μl of dye. Line 7 -> 3 μl of plasmid J23106A + 1 μl of dye. Line 8 -> 3 μl of plasmid J23106B + 1 μl of dye. Line 9 -> 3 μl of plasmid J23106C + 1 μl of dye. Line 10 -> 3 μl of plasmid J23106D + 1 μl of dye. Line 11 -> 3 μl of plasmid J23107A + 1 μl of dye. Line 12 -> 3 μl of plasmid J23107B + 1 μl of dye. Line 13 -> 3 μl of plasmid J23107C + 1 μl of dye. Line 14 -> 3 μl of plasmid J23107D + 1 μl of dye. Line 15 -> 3 μl of plasmid J23108A + 1 μl of dye. Gel 13. Plasmid (15 lines) Line 1 -> 3 μl of plasmid J23108B + 1 μl of dye. Line 2 -> 3 μl of plasmid J23108C + 1 μl of dye. Line 3 -> 3 μl of plasmid J23108D + 1 μl of dye. Line 4 -> 3 μl of plasmid J23109A + 1 μl of dye. Line 5 -> 3 μl of plasmid J23109B + 1 μl of dye. Line 6 -> 3 μl of plasmid J23109C + 1 μl of dye. Line 7 -> 3 μl of plasmid J23109D + 1 μl of dye. Line 8 -> 3 μl of plasmid J23119B + 1 μl of dye. Line 9 -> 3 μl of plasmid J23119C + 1 μl of dye. Line 10 -> 3 μl of plasmid J23119D + 1 μl of dye. Line 11 -> 3 μl of plasmid J23106B + 1 μl of dye. Line 12 -> 3 μl of plasmid J23108C + 1 μl of dye. Line 13 -> 3 μl of plasmid J23113C + 1 μl of dye. (gel 8) Line 14 -> 3 μl of plasmid J23117B + 1 μl of dye. (gel 9) Line 15 -> Green ladder.