Interchanging Parts Information
Hi Maria and Edinburgh team!
It was very nice to receive news from you. We really hope that despite the
actual situation with your reduced team, everything works out with your
project. In our experience, we now know that working in an iGEM project
is a huge challenge, there is always a lot of work to do but we are always
trying to do our best and we are sure that all of you are also doing the
same.
Well, about our parts you can find more detailed information at the
following link:
http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UNAM-Genomics_Mexico
The three next parts were synthesized and they don´t have any promoter. At
the ends they have the prefix and suffix sequences. Their sequences are
those reported in the registry page aforementioned, but I am sending the
Mr. Gene sequences for any possible delay in our annotation.
1. PCR amplified product containing green light photosensor CcaS and CcaR
response regulator.
>projectID|14435| csar_csas_noscar_rbs_pANY (n/a / n/a)
GAATTCGCGGCCGCTTCTAGAGTCACACAGGAAAGAACAGCTATGGGCAAATTTCTAATTCCAATCGAATTTGTTTTTCT
GGCGATCGCCATGACCTGTTATTTATGGCACAGACAAAACCAAGAACGCCGCAGGATTGAAATTAGCATCAAGCAACAAA
CCCAACGGGAACGATTTATTAACCAAATTACCCAACATATCCGCCAATCTTTAAACTTGGAAACGGTTTTAAATACCACC
GTCGCTGAAGTTAAAACCCTGTTGCAAGTTGATCGAGTTCTAATTTATCGCATTTGGCAAGATGGCACGGGCAGCGCCAT
TACGGAATCGGTGAATGCCAATTATCCTAGTATTTTAGGGCGGACCTTTTCCGATGAAGTTTTTCCCGTTGAATACCATC
AAGCCTACACCAAAGGTAAAGTACGGGCCATTAATGACATTGACCAGGATGACATAGAGATTTGCCTAGCTGATTTCGTC
AAACAATTTGGCGTGAAATCAAAATTAGTAGTGCCCATTCTTCAACATAATCGTGCTTCTTCCCTAGATAATGAATCAGA
ATTTCCCTATCTTTGGGGGCTGTTAATTACCCATCAATGTGCTTTTACCCGGCCATGGCAACCGTGGGAAGTGGAGTTAA
TGAAACAGCTAGCCAATCAGGTCGCGATCGCCATCCAACAATCGGAATTATATGAGCAATTACAGCAACTCAATAAAGAT
TTGGAAAACCGAGTCGAAAAACGCACCCAGCAACTTGCCGCCACCAATCAATCCCTAAGAATGGAAATCAGTGAGCGACA
AAAAACGGAAGCCGCTCTCCGCCACACTAACCATACTCTGCAATCCCTGATTGCGGCCTCCCCCAGGGGTATTTTTACCC
TTAATTTAGCAGACCAAATTCAGATTTGGAATCCTACAGCAGAACGTATTTTTGGTTGGACAGAAACAGAAATTATTGCC
CATCCAGAATTATTAACATCCAACATTTTGCTGGAAGATTATCAGCAATTTAAACAGAAAGTTTTATCAGGCATGGTTTC
CCCTAGCCTAGAATTAAAATGTCAAAAAAAAGATGGTAGTTGGATTGAAATTGTCCTTTCCGCTGCTCCCCTATTGGATA
GTGAAGAAAATATTGCCGGATTGGTGGCGGTTGTCGCCGATATTACCGAGCAAAAGCGGCAGGCAGAACAAATTCGTTTG
CTACAATCCGTTGTGGTTAATACTAATGATGCGGTGGTGATTACGGAAGCGGAGCCCATTGATGATCCCGGGCCGAGAAT
ACTCTATGTCAATGAAGCATTTACTAAAATCACCGGTTATACTGCTGAAGAAATGCTAGGCAAAACCCCCCGAGTTTTAC
AGGGACCAAAAACCAGTCGCACTGAATTAGATAGGGTGCGGCAAGCCATTAGTCAATGGCAATCAGTTACCGTTGAAGTG
ATTAATTATCGTAAGGATGGCAGTGAGTTTTGGGTGGAATTTAGTCTGGTGCCCGTTGCCAATAAAACAGGTTTTTACAC
CCATTGGATTGCTGTGCAAAGGGATGTCACTGAGCGCCGACGCACGGAGGAAGTCCGCCTAGCTTTAGAACGGGAAAAAG
AATTAAGCCGCCTAAAAACTCGTTTTTTCTCCATGGCTTCCCATGAATTTCGTACTCCCCTCAGTACGGCCTTAGCTGCT
GCCCAATTACTGGAAAATTCTGAAGTGGCCTGGCTTGATCCCGATAAGCGTAGCCGGAACTTACACCGTATTCAAAATTC
CGTGAAAAATATGGTACAGCTCCTGGATGATATTTTAATCATTAACCGTGCCGAAGCGGGCAAATTGGAATTTAATCCTA
ATTGGTTAGATTTGAAATTATTGTTCCAGCAATTTATCGAAGAAATTCAATTAAGTGTCAGTGACCAATATTATTTTGAC
TTTATTTGTAGCGCTCAAGATACGAAGGCATTGGTGGATGAAAGGTTAGTGCGGTCTATTTTATCTAATCTGTTATCTAA
TGCGATTAAATACTCTCCCGGGGGAGGGCAGATTAAAATTGCCCTAAGCCTAGATTCGGAACAGATTATTTTTGAAGTCA
CCGACCAGGGCATTGGCATTTCGCCAGAGGACCAAAAGCAAATTTTTGAACCCTTTCATCGGGGCAAAAATGTCAGAAAT
ATTACGGGAACAGGACTCGGTTTAATGGTTGCCAAGAAATGTGTTGACTTACACAGTGGCAGTATCTTGCTAAAAAGTGC
AGTTGACCAGGGAACAACAGTTACTATCTGTTTAAAACGCTATAACCATTTGCCTCGAGCTTAGTAATAATACTAGAGAC
TTTAAGCACAGGAAAGATATCATATGAGAATACTTTTAGTGGAGGATGATTTGCCGCTGGCGGAAACCCTTGCTGAAGCA
TTGAGTGACCAGCTTTACACCGTTGATATTGCCACCGACGCTTCCCTCGCCTGGGACTATGCCTCCCGACTGGAATATGA
CCTCGTTATTTTGGATGTGATGCTGCCGGAGTTGGACGGGATTACCCTCTGTCAAAAATGGCGATCGCACAGTTATTTAA
TGCCAATTTTGATGATGACAGCCAGGGATACGATCAATGATAAAATCACGGGCTTGGATGCGGGGGCGGATGATTATGTG
GTCAAGCCAGTGGATTTGGGGGAGTTATTTGCCAGGGTGCGAGCTTTGTTGCGTCGGGGTTGTGCAACGTGCCAACCAGT
TTTAGAGTGGGGGCCAATCAGGTTGGACCCAAGCACCTATGAAGTTAGTTATGACAATGAGGTTTTGTCTTTGACCCGCA
AGGAATACAGCATTCTGGAATTACTACTCCGCAATGGCCGTCGGGTGCTAAGTCGGAGCATGATTATCGATAGTATCTGG
AAGTTGGAGAGTCCCCCAGAGGAAGATACGGTTAAGGTGCATGTGCGGAGTTTGCGACAAAAATTAAAAAGTGCCGGTTT
ATCAGCAGATGCCATTGAAACGGTCCATGGCATTGGGTATCGTCTGGCCAATTTAACGGAAAAATCTTTGTGCCAAGGGA
AAAACTAGTAATAACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGT
CGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCT
GCAG
IMPORTANT: This sequence was modified because it had a SpeI restriction
site, thus the PCR product that we sent you is shorter. The sequence
corrected is the one reported at the registry page and includes the prefix
and suffix sequences in the ends.
We did't have time to purify this PCR reaction, so that it has a label as
not cleaned PCR product, but the other PCR reactions were correctly
purified.
2. PCR amplified product containing blue light photosensor LovTAP. Full
length 889 nt, including the prefix and suffix sequences.
>projectID|14201| 2_LovTap_pANY (n/a / n/a)
GCGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAAGTACTATGTTGGCTACTACACTTGAACGTATTGAGAAGAACTTT
GTCATTACTGACCCAAGGTTGCCAGATAATCCCTTTATATTCGCGTCCGATAGTTTCTTGCAGTTGACAGAATATAGCCG
TGAAGAAATTTTGGGAAGAAATTGTCGTTTTCTACAAGGTCCTGAAACTGATCGCGCGACAGTGAGAAAAATTAGAGATG
CCATAGATAACCAAACAGAGGTCACTGTTCAGCTGATTAATTATACAAAGAGTGGTAAAAAGTTCTGGAACCTCTTTCAC
TTGCAGCCTATGCGAGATCAGAAGGGAGATGTCCAGTACTTTATTGGGGTTCAGTTGGATGGAACTGAGCATGTCCGAGA
TGCTGCCGAGAGAGAGGGAGTCATGCTGATTAAGAAAACCGCCGAAAATATTGATGAGGCGGCATTTGTCGACCTGCTTA
AGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACT
CGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCGGGCATCGC
GACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTGGAGCTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAA
GCGATTAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTG
TTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG
CCGCTGCAG
3. PCR amplified product containing a YFP protein, LuxY. Full length 820
nt, including the prefix and suffix sequences.
>projectID|14198| 2_LuxY_pANY (n/a / n/a)
GCGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAAAGAGGAAACAGCTATGTTT
AAAGGTATAGTAGAAGGTATAGGAATCATTGAAAAAATTGATATATATACTGACCTAGATAAGTATGCAATTCGATTTCC
TGAAAATATGTTGAATGGAATTAAAAAGGAGTCGTCAATAATGTTTAACGGATGCTTCTTAACGGTAACTAGCGTAAATT
CAAACATTGTCTGGTTTGATATATTTGAAAAAGAAGCACGTAAGCTTGATACTTTTCGGGAATATAAGGTAGGTGACCGA
GTAAATTTAGGAACATTCCCAAAATTTGGCGCTGCATCTGGTGGGCATATATTATCAGCAAGGATTTCATGTGTAGCAAG
TATTATTGAAATAATAGAAAATGAGGATTATCAACAAATGTGGATTCAAATTCCTGAAAATTTTACAGAGTTTCTTATTG
ATAAAGACTATATTGCTGTGGATGGTATTAGCTTAACTATTGACACTATAAAAAACAACCAATTTTTCATTAGTTTACCC
TTAAAAATAGCACAAAATACAAATATGAAATGGCGAAAAAAAGGTGATAAGGTAAATGTTGAGTTATCAAACAAAATTAA
TGCTAACCAGTGTTGGTAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGT
TTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATAT
ACTAGTAGCGGCCGCTGCAG
The LuxAB genes were obtained from Vibrio Fischeri Genomic DNA, the
product include the prefix and suffix sequences.
Its sequence is at the registry page, but if further information is needed
please contact to Augusto and Daniela.
The information about the LovTAP ligations with promoters (J23117, J23105,
J23114 and J23102) inside plasmid J61002 can be found at the next link:
http://openwetware.org/wiki/IGEM:UNAM/2009/Notebook/Modeling_logbook_Claudia/2010/08/26
The information of the blue light promoter will be updated today at the
registry page.
If there are any questions, please contact us.
According to the information that you sent us, we are very interested in
receive the following parts:
-LovTap readout system.
-PCB production plasmid.
-luxCDE genes.
We would like to have more information about the red Firefly luciferase
mutation - Is it functional? if so, we would also wanted to receive it,
despite that it wasn´t as a registry-standard part.
About the other parts that you mentioned- red light sensor and the fuse
luxAB with lumP- they would be also very useful but if your team is still
working on them maybe they could be sent later.
Thank you so much for stay in touch with us.
Regards and Good Luck!
UNAM-Genomics-Mexico Team
| 
WiFi Coli Collaborations
Main project page
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