Biofilm Track: OD Growth Curve
Obtained ON cultures from ambl at 10:30 am (18.5 hours aerated in 32.5C, 180rpm)
- Prepared culture mix: 25ml B2 broth + 1ml ON culture for growth curve test
Errors:
- Didn't do 0 time reading: Measurement right after inoculating 1 ml staph into 25ml B2 broth
- Didn't have growth control while swirling culture flask (B2+staph) in 37 shaker
- All values are way to high than normal: For OD 600; We want reading around 0.4 for last reading point (3-5 hours after)
Next Step: Redo Growth Curve Experiment
- Prepared new ON cultures from glycerol stock: (3ml LB + staph), (3ml B2 +staph), (3ml B2 growth control), (3ml LB growth control)
- Placed new ON tubes into 37 shaker (90rpm) at 4:30pm; To be removed by 10:30pm (Saturday, July 3rd)
Growth Curve Experiment
| Time | Sample Number | OD reading 1 (A) | OD Reading 2 (A) | OD reading 3 (A) |
| | 12:10-1:10pm | 1 | 1.585 | 1.580 | - |
| | 1:10-1:40pm | 2 | 2.454 | 2.468 | 2.450 |
| | 1:50-2:20pm | 3 | 4.396 | 4.426 | 4.384 |
| | 2:20-2:50pm | 4 | 5.144 | 5.174 | 5.166 | |
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iGEM Project name 1
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