IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/16

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Thursday 16th September

  • Ligations were set up between the promoter-GFP and the digested pSB1C3 plasmid (which was retrieved from part BBa_J04450) using the method shown bellow:
Substance (μL) Tube 1 Tube 2 Tube 3 Tube 4
pSB1C3 1 2 5 2
Promoter 1 5 2 2
Ligase 1 1 1 0
Buffer 1 1 1 1
H2O 6 1 1 5


  • These ligations were left for 2 hours then using 5μL they were transformed into chemically competent DH5 alpha cells.