IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/16

iGEM Project name 1

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Thursday 16th September Ligations were set up between the promoter-GFP and the digested pSB1C3 plasmid (which was retrieved from part BBa_J04450) using the method shown bellow: Substance (μL) Tube 1 Tube 2 Tube 3 Tube 4 pSB1C3 1 2 5 2 Promoter 1 5 2 2 Ligase 1 1 1 0 Buffer 1 1 1 1 H2O 6 1 1 5 These ligations were left for 2 hours then using 5μL they were transformed into chemically competent DH5 alpha cells.