IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/15

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM Project name 1 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Wednesday 15th September

  • The overnight digestions were checked using gel electrophoresis[[1]]. Which was set up as shown bellow:
  • Starting from the top:
 Lane 1) BBa_J04450
 Lane 2) Ladder
 Lane 3) BBa_J04450
 Lane 4) HapR-GFP
 Lane 5) CsrB-GFP
 Lane 6) pgaA-GFP
 Lane 7) CsrB-GFP
 Lane 8) pgaA-GFP


  • The small band from the lanes containing a cut promoter-GFP were extracted using the gel extraction kit.[[2]]
  • The large Band from the lanes containing part BBa_J04450 were extracted using the gel extraction kit.[[3]]