IGEM:Peking University/2008/Notebook/Group 2/2008/06/21

From OpenWetWare
Jump to: navigation, search
PKU logo08 01.jpg Group 2 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

0621

TITLE: PCR-pPT1-Gal4-Phusion 50ul*4
Volume (μL)
Template 0.5
dNTP 2
Enzyme 0.5
Buffer 10
F-Primer 0.2
R-Primer 0.2
ddH2O 36.6
conditions
Temperature Time Length(0:00:00)
98°C 01:30
98°C 00:10 30 cycles
60.6,63.1,64.5,68.2°C 00:30 30 cycles
72°C 01:30 30 cycles
72°C 07:00
4°C Hold

TITLE: PCR-Budding Yeast Genome-Gal4-Phusion 50ul*4
Volume (μL)
Template 0.5
dNTP 2
Enzyme 0.5
Buffer 10
F-Primer 0.2
R-Primer 0.2
ddH2O 36.6
conditions
Temperature Time Length(0:00:00)
98°C 01:30
98°C 00:10 30 cycles
60.6,63.1,64.5,68.2°C 00:30 30 cycles
72°C 01:30 30 cycles
72°C 07:00
4°C Hold

TITLE: PCR-pPT-Gal4-Transtart 20ul*6
Volume (μL)
Template 0.5
dNTP 2
Enzyme 0.5
Buffer 2
F-Primer 0.2
R-Primer 0.2
ddH2O 14
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold

Ligation-pEasy-T1/Gal4-E.Coli-Amp+,K+(BlueWhiteSelection)