0621
TITLE: PCR-pPT1-Gal4-Phusion 50ul*4
|
Volume (μL)
|
Template |
0.5
|
dNTP |
2
|
Enzyme |
0.5
|
Buffer |
10
|
F-Primer |
0.2
|
R-Primer |
0.2
|
ddH2O |
36.6
|
- conditions
Temperature |
Time Length(0:00:00)
|
98°C |
01:30
|
98°C |
00:10 |
30 cycles
|
60.6,63.1,64.5,68.2°C |
00:30 |
30 cycles
|
72°C |
01:30 |
30 cycles
|
72°C |
07:00
|
4°C |
Hold
|
TITLE: PCR-Budding Yeast Genome-Gal4-Phusion 50ul*4
|
Volume (μL)
|
Template |
0.5
|
dNTP |
2
|
Enzyme |
0.5
|
Buffer |
10
|
F-Primer |
0.2
|
R-Primer |
0.2
|
ddH2O |
36.6
|
- conditions
Temperature |
Time Length(0:00:00)
|
98°C |
01:30
|
98°C |
00:10 |
30 cycles
|
60.6,63.1,64.5,68.2°C |
00:30 |
30 cycles
|
72°C |
01:30 |
30 cycles
|
72°C |
07:00
|
4°C |
Hold
|
TITLE: PCR-pPT-Gal4-Transtart 20ul*6
|
Volume (μL)
|
Template |
0.5
|
dNTP |
2
|
Enzyme |
0.5
|
Buffer |
2
|
F-Primer |
0.2
|
R-Primer |
0.2
|
ddH2O |
14
|
- conditions
Temperature |
Time Length(0:00:00)
|
94°C |
02:00
|
94°C |
01:00 |
4 cycles
|
50°C |
02:00 |
4 cycles
|
72°C |
05:00 |
4 cycles
|
94°C |
01:00 |
30 cycles
|
55.2,55.6,57.6,59.5,61.3,63.9°C |
01:00 |
30 cycles
|
72°C |
03:00 |
30 cycles
|
72°C |
10:00
|
4°C |
Hold
|
- Ligation-pEasy-T1/Gal4-E.Coli-Amp+,K+(BlueWhiteSelection)
|