IGEM:Peking University/2008/Notebook/Group 2/2008/06/23

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0623

TITLE: PCR-pPT-Gal4-Transtart 50ul*2
Volume (μL)
Template 1
dNTP 5
Enzyme 0.5
Buffer 5
F-Primer 0.5
R-Primer 0.5
ddH2O 37.5
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
57.4,58.8°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold