IGEM:Peking University/2008/Notebook/Group 2/2008/06/03

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PCR

TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6
Volume (μL)
Template (Budding Yeast Genome) 1
dNTP 2
Enzyme 0.5
Buffer 2
F-Gal4-HindIII-ApaI 0.5
R-Gal4-EcoRI-Xhol 0.5
ddH2O 13.5
conditions
Temperature(&&&) Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 00:30 4 cycles
94°C 01:00 30 cycles
52.0,53.6,56.1,58.8,60.1,62.0°C 02:00 30 cycles
72°C 04:00 30 cycles
72°C 05:00
4°C Hold


TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3
Volume (μL)
Template (Budding Yeast Genome) 1
dNTP 5
Enzyme 1
Buffer 5
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol 1
ddH2O 36
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 00:30 4 cycles
94°C 01:00 30 cycles
54.7,57.4,60.1°C 02:00 30 cycles
72°C 04:00 30 cycles
72°C 05:00
4°C Hold