IGEM:Peking University/2008/Notebook/Group 2/2008/05/28

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PCR

TITLE: PCR-Budding Yeast Genome-Gal4-K.O.D 50ul
Volume (μL)
Template (Budding Yeast Genome) 1
dNTP 5
Enzyme 1
Buffer 5
MgSO4(For KOD) 2
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol 1
ddH2O 34
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 00:20 30 cycles
59°C 00:30 30 cycles
55°C 00:30 30 cycles
68°C 03:00 30 cycles
68°C 07:00
4°C Hold

Failed.