IGEM:Peking University/2008/Notebook/Group 1/2008/07/26

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PKU_080726_G1_1



Enzyme cutting-(TSH)-pGREG504-pADH-lac1-1/1-2/S-1/S-2/tet-1/tet-2


Enzyme cutting-AID(PCR product)


Gel extraction and ethanol precipitation


Ligation-pADH-lac1-1/1-2/S-1/S-2/tet-1/tet-2-AID


Transformation




Dissolving DNA from iGEM Biobrick


Transformation



Miniprep-pADH-lac1-1-1/2-2-1/2-2-2/2-2-3/S-1-1/S-2-1


Digestion-pADH-lac1-1-1/2-2-1/2-2-2/2-2-3/S-1-1/S-2-1-ClaI-BamHI


Gel running and testing

Result:lanes for pADH-lac1-1-1/2-2-1/2-2-2/S-2-1 have 700bp bands but weak; other two not.