IGEM:Peking University/2008/Notebook/Group 1/2008/07/25

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PKU_080725_G1_1



Picking up colonies and grow in LB+A+-6 in all


Enzyme cutting-lac1/2/S/tet (redo)
File:PKU-080725-xili plasmid test-redigest-1.tif

lanes:1kb, 100bp, lac1, lac2-1, lac2-2, lacS, tet, lambda marker

Result: no 1.4kb bands; lac1:100-1000bp smear; lac2:700bp band; lacS:700bp band, 1.4kb smear, high concentration; tet: 100-1500bp high concentration, smear.


Volume (μL)
plasmid 3
ClaI 0.2 ×5
BamHI 0.2
1 × K Buffer 2
ddH2O 14.6


Transformation-pADH-lac1/2/S/tet (redo)