IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/06/08

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Date: 6/8/14 People in lab: Caleb Treccazi

Title: Chemical Transformation of 6/6 L1 and L2 Start Time: 10:30 am Purpose: To check for successful product from ligation Protocol: LTM Ed. 2 Chemical Transformation Exceptions: Products:

Sample Label Description Source Label Quantity
CT 1 6/8 norV A1 and chlor R norV gene with chlor resistant backbone L 1 6/6 1
CT 2 6/8 norV A2 and chlor R norV gene with chlor resistant backbone L 2 6/6 1
CT 3 negative control Dead cells Competent cells and chlor plate 1
CT 4 6/8 positive control Live cells Comp cells and amp plate 1

Results: no growth besides on the positive control plate where there were very few colonies Notes: Old comp cells were used that were found in the back of the -80 C freezer; cell competency is suspect and experiment needs repeating with new competent cells and new plates Stop Time: 1:30 pm Next: Repetition with new materials