IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/06/07
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Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb TreccaziTitle: PCR of hmp gene Start Time: 10:30 am Purpose: To add the iGEM prefix and suffix Protocol: LTM Ed. 2 PCR Exceptions: Products:
Results: Notes: Stop Time: 11 am Next: Gel electrophoresis Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb TreccaziTitle: Gel electrophoresis of hmp PCR products Start Time: 11:30 am Purpose: To check for products Protocol: LTM Ed. 2 Gel Exceptions: Products:
Products: Results: Notes: Stop Time: 12: 15 pm Next: TOPO Date: 6/7/14 People in lab: Emily Puleo, Kira Buckowing, Caleb TreccaziTitle: TOPO cloning of hmp Start Time: 11:45 am Purpose: To make more copies of amplified hmp Protocol: LTM Ed. 2 Chemical Transformations and Invitrogen TOPO cloning kit manual Exceptions: Products:
Results: (6/8) No growth seen - cells not competent or died? Notes: Stop Time: 3:30 pm Next: Make competent cells (ran out); try PCR once more
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