IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/30

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Entry title Order: S+I(1,2); S+I(3,4) Gel run with entire template: NO PCR product found PCR of S+I7 with primers 1 and 2 PCR program: 1) 98°C for 2min; 2) 98°C for 30s; 3) 64.4°C for 30s; 4) 72°C for 45s; go to 2 for 30 times 5) 72°C for 30min; 6) 4°C hold inoculated DH5alpha in SOB for competant cell preparation.