IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/18

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Entry title

  • 4 gel runs for all the minipreps
  • GEL 1 Order:
  • ROW 1: Ladder; 001(A2); 001(A4); 001(A5 through 7); BLANK; Ladder; 002(A1 through 7)
  • ROW 2: BLANK; 003(A1 through 7); Ladder; 004(A2 through 7)
  • GEL 2 Order (Digested parts):
  • ROW 1: 005 (A1 through 7); ladder; 006(A1 through 7)
  • ROW 2: 998(A1 through 7); Ladder; 999(A1 through 7)
  • GEL 2 Order (Undigested parts):
  • ROW 1: 005 (A1 through 7); ladder; 006(A1 through 7)
  • ROW 2: 998(A1 through 7); Ladder; 999(A1 through 7)
  • Construction of the following 8 composite parts: [these were clubbed as B set of Ligations]
  • J23119 + S03879 ------> 001
  • B0034 + K143065 ------> 002
  • B0034 + K145151 ------> 003
  • B0014 + J23119 -------> 004
  • B0034 + K156011 ------> 005
  • R0010 + S03879 -------> 006
  • R0040 + E0420 --------> 500
  • R0010 + E0420 --------> 502




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