IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/14

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Entry title

  • Plasmid miniprep (pSB1A3; pSB4A5; pSB1C3 and pSB4C5) done.
  • Construction of the following composite parts using 3A assembly in pSB1C3 backbone [grouped as A set of Ligations]
  • J23119 + S03879 -------> 001
  • B0034 + K143065 -------> 002
  • B0034 + K145151 -------> 003
  • B0014 + J23119 --------> 004
  • B0034 + K156011 -------> 005
  • R0010 + S03879 --------> 006
  • R0010 + S0104 ---------> 998
  • J23119 + S0104 --------> 999
  • Digestions:
  • Upstream parts: E/S
  • Downstream parts: X/P
  • Plasmid: E/P