IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/14
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Plasmid miniprep (pSB1A3; pSB4A5; pSB1C3 and pSB4C5) done.
Construction of the following composite parts using 3A assembly in pSB1C3 backbone [grouped as A set of Ligations]
J23119 + S03879 -------> 001
B0034 + K143065 -------> 002
B0034 + K145151 -------> 003
B0014 + J23119 --------> 004
B0034 + K156011 -------> 005
R0010 + S03879 --------> 006
R0010 + S0104 ---------> 998
J23119 + S0104 --------> 999
Digestions:
Upstream parts: E/S
Downstream parts: X/P
Plasmid: E/P
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