IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/12

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  • Miniprep done for the 17 parts.
  • Ultra competent cells of DH5a and DB3.1 prepared and stored at -80 degrees Celsius.
  • OD for Dh5a was 0.4 and 0.9 for DB3.1 (protocol states that it should be about 0.3 to 0.5); LB was used as blank whereas media used for inoculation of cells was SOB.
  • Transformation of DB3.1 with the following 5 Plasmid backbones :
  • pSB1A3
  • pSB1C3
  • pSB3C5
  • pSB4A5
  • pSB4C5
  • Also transformed the following parts
  • K117005
  • B0034
  • Poured Agar Plates (Amp and Chl)