IGEM:IMPERIAL/Protocols

Protocols

• Bacterial Culture
• LB Amp Plate
• Transformations
• Competent Cells
• Bradford Assay
• 20 uL Digestion
• 20 uL Ligation
• 30 uL Ligation
• Miniprep of Culture
• 50x TAE Solution
• Agarose Gel
• Measuring DNA Concentration
• Fusion PCR protocol
• Western Blot Protocol
• Protocal for aiia PCR
• 1 kb Plus DNA Ladder
• Freezing cells (Protocol from OWW: Making a long term stock of bacteria)

• PCR v. miniprep (flow chart)

Part Testing Protocols

• T9002/J37016/J37020 Testing Protocol
• J37015 Testing Protocol
• J37015-LuxI Testing Protocol PRELIMINARY VERSION
• I13207 Testing Protocol UNFINISHED (This part is no longer necessary)
• J37017 Testing Protocol NOT NEEDED
• J37022 Testing Protocol
• S01656 Testing Protocol
• New S0165 testing protocal

Biosensor Testing Protocol

• Biosensor Testing: Enzyme Activity
• Biosensor Testing: Calibration & Characterization