IGEM:IMPERIAL/2007/Week5

For the Weekend

Wet lab: Cheuk, Maira, Ben
- Cloning:
  1. sort out DNA constructs to build
  2. transform cells
  3. amplify biobricks
  4. midi/maxi preps
  5. digestion and ligation
  6. build complete DNA constructs
- In vitro:
  1. wait for materials/look for BL21
  2. prepare cell extracts

By end of week: Build complete DNA constructs

Merge protocols: James, Peixuan, Lucas
- Define standard units
- Learn chassis
- Learn cell-by-date
- Learn biofilm
  1. merege protocols (prioritise)
  2. provisional lab schedule
  3. learn biofilm production

By end of week: Efficient planning pf protocols and lab sessions

Learn vessicles: Dirk, Cheuk
- Read up papers
  1. materials
  2. equipment
  3. compare results of different techniques
- Contact people
  1. focus on techniques
  2. ask for opinion

By end of this week: Finalise protocols, Wait for material delivery

Wiki: Everyone
- Have a schedule
  1. weekly
  2. daily
- Updated project page
  1. progress throughout each day
  2. progress throughout each week
- Have an iGEM 'digest' for the professors and supervisors (email)

By end of week: Get supervisors back into the 'loop'. Get organised

Modelling: Jerry, Alex
- Learn biofilm
  1. diffusion process within biofilms
  2. [AHL] and temperature dependence
- Learn cell-by-date
  1. temperature dependence
  2. degradation time
  3. steady state (rate of formation/decay of GFP)

By end of week: Model basic constructs and parameters to be measured