IGEM:IMPERIAL/2007/Projects/Hrp System/Systems/Hrp Device 2/TestingValidation

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Super Parts links to higher level parts if applicable
Actual Part
IC2007 Hrp Dev2Top.png
Sub Parts RBS (Elowitz) BBa_B0034 Double terminator (B0010-B0012) BBa_B0015



Equipment

  • Twinkle Plate Reader Fluorometer
  • 96 well plate
  • Eppendorf Tubes
  • Gilson Pippettes
  • Plastic Curvetts
  • Incubator 37o shaker
  • Vortex and Centrifuge
  • Spectrometer

Materials and Reagents

  • M9 minimal media 40μg/ml Ampicillin
  • Inducer of promoter at varying concentrations
  • Recombinant acGFP of known concentration
  • Agar Plates
  • E.coli strain containing vector


Protocol

Device 2 Protocol Two promoters are tested each have a range of 8 inducer concentrations.

  • pBad will have a range of 0 to 100mM added at intervals of 12.5mM Protocol
  • pLac will have a range of 0 to 0.1mM IPTG added at intervals of 0.0125mM. Protocol

Device Test Construct

For this test construct we initially need to run preliminary tests in order to find out what time intervals and what input ranges we need. To run this the range of concentrations used for promoters will be used. This device uses a pLac promoter, the proposed range of concentrations is going to be defined from the promoter experiment, this is use a range of concentrations to cover an appropriate range of promoter. From looking at literature an appropriate range is 0-100μM.