IGEM:IMPERIAL/2007/Projects/Hrp System/Systems/Hrp Device 2

Super Parts	links to higher level parts if applicable
Actual Part
Sub Parts	RBS (Elowitz) BBa_B0034	Double terminator (B0010-B0012) BBa_B0015

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Motivations

• Device simulates a basic AND gate with one inverted input.
• HrpR and HrpS are controlled by a single promoter serving as the excitatory input to HrpL.
• HrpV expression is dependent on a different promoter and serves as an inhibitory input.

Part interface

INPUTS OUTPUTS PoPS (HrpV strand) PoPS (pHrpL) PoPS (HrpR & HrpS)

Expected behavior and performances

(try to write your specifications in tables. Here are just 2 examples)

Interface specifiactions

Characteristics	Input 1	Input 2	Input 3	Output 1	Output 2
Range	[X1,Y1]	[X2,Y2]	[X3,Y3]	[X4,Y4]	[X5,Y5]
Tolerance	T1	T2	T3	T4	T5
Transfer function	n/a	n/a	n/a	insert image	insert image
Latency

System level specifications

Properties	Comments
Range
Transfer function
Latency
Robustness
Variability
properties 3
properties 4

Open issues

• list known issues