IGEM:IMPERIAL/2007/Projects/Hrp System/List of Required Parts

Hrp Characterisation: Design (Required Parts)

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

This is the list of required parts that can be obtained from the registry and are needed for the project.

Promoters

List input promoters, find/measure PoPS in of the following promoters:

• LuxR Promoter

BBa_F2620

Short description: A transcription factor [LuxR] (C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as aTc can be used to produce a logical AND function.

• Constitutive promoter family

BBa_J23119

Short desccription: Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. Part J23119 is present in pSB1A2.

• Promoter P(Lac) IQ

BBa_I14032

Short description: Constitutive Promoter, High Transcription

• Promoter (tetR, negative)

BBa_R0040

Short description: Medium strength promoter. Sequence for pTet inverting regulator driven by the TetR protein. Action is inhibited by the addition of tetracycline or its analog, aTc.

• Promoter (lambda cI regulated)

BBa_R0051

Short description: The cI regulated promoter is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).

• CMV promoter

BBa_J52034

Short description: CMV promoter with RBS region, used for testing of our reporter constructs. CMV promotor is a constitutive promotor.

Ribosome Binding Sites

• RBS (Elowitz)

BBa_B0034

Short description: This is the most efficient RBS in the registry, and sets the 1.0 efficiency standard. It is based on the Elowitz repressilator.

Terminators

• Double terminator (B0010-B0012)

BBa_B0015

Short description: Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. forward_efficiency: 0.984 reverse_efficiency: 0.295

Parts Not in the Registry (To Characterize)

• HrpR
• HrpS
• HrpV
• pHrpL

Further parts:

• σ_L (HrpL)
• Hrp Box