anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline: Prepare a 1,000 times stock solution: 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.