IGEM:IMPERIAL/2007/Projects/Hrp System/Devices

Hrp Characterisation: Devices

• Introduction
• Specifications
• Parts
• Devices
• Testing
• Application
• Notes
• References

Characterisation

This is a prioritised list of devices utilising the Hrp system that we would like to characterise.

Device 1: One input

Follower: pHrpL promoter - RS (single operon) only

This device is a pHrpL promoter utilising a single RS operon only.
A single promoter activates HrpR and HrpS expression simultaneously.

Device 2: Two inputs

AND with one inversion: pHrpL promoter - RS and V

This device simulates a basic AND gate with one inverted input.
Again, HrpR and HrpS are controlled by a single promoter. HrpV expression is
dependent on a different promoter. The R and S proteins activate the pHrpL
promoter while the V protein represses it.