IGEM:IMPERIAL/2007/CFS/References

Cell-Free Systems

• Introduction
• Specifications
• Design
• Fabrication
• Testing
• Validation
• Notes
• References

References

E. coli Cell Extracts

1. Kim TW, Keum JW, Oh IS, Choi CY, Park CG, and Kim DM. Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J Biotechnol. 2006 Dec 1;126(4):554-61. DOI:10.1016/j.jbiotec.2006.05.014 |

In Vitro Protein Synthesis

1. In vitro translation (Ambion)
2. In vitro protein expression (Promega)
3. Cell extract user guide(Promega)
4. EasyXpress protein synthesis mini kit (Qiagen)

Cell-Free Expression Systems

1. Noireaux V and Libchaber A. A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17669-74. DOI:10.1073/pnas.0408236101 |
2. Noireaux V, Bar-Ziv R, Godefroy J, Salman H, and Libchaber A. Toward an artificial cell based on gene expression in vesicles. Phys Biol. 2005 Sep 15;2(3):P1-8. DOI:10.1088/1478-3975/2/3/P01 |
3. Pellinen T, Huovinen T, and Karp M. A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal Biochem. 2004 Jul 1;330(1):52-7. DOI:10.1016/j.ab.2004.03.064 |
4. Fletcher MP, Diggle SP, Cámara M, and Williams P. Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules. Nat Protoc. 2007;2(5):1254-62. DOI:10.1038/nprot.2007.158 |
5. Noireaux V, Bar-Ziv R, and Libchaber A. Principles of cell-free genetic circuit assembly. Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12672-7. DOI:10.1073/pnas.2135496100 |

Purified Cell-Free Systems

1. Cell-free translation systems for protein engineering by Yoshihiro Shimizu et al (2006)
2. Efficient protein selection based on ribosome display system with purified components by Hiroyuki Ohashi et al (2006)

Pore Proteins for Cell-Free Exchange

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