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Cell-Free Systems


E. coli Cell Extracts

  1. Kim TW, Keum JW, Oh IS, Choi CY, Park CG, and Kim DM. Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J Biotechnol. 2006 Dec 1;126(4):554-61. DOI:10.1016/j.jbiotec.2006.05.014 | PubMed ID:16797767 | HubMed [1]

In Vitro Protein Synthesis

  1. In vitro translation (Ambion)
  2. In vitro protein expression (Promega)
  3. Cell extract user guide(Promega)
  4. EasyXpress protein synthesis mini kit (Qiagen)

Cell-Free Expression Systems

  1. Noireaux V and Libchaber A. A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17669-74. DOI:10.1073/pnas.0408236101 | PubMed ID:15591347 | HubMed [1]
  2. Noireaux V, Bar-Ziv R, Godefroy J, Salman H, and Libchaber A. Toward an artificial cell based on gene expression in vesicles. Phys Biol. 2005 Sep 15;2(3):P1-8. DOI:10.1088/1478-3975/2/3/P01 | PubMed ID:16224117 | HubMed [2]
  3. Pellinen T, Huovinen T, and Karp M. A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal Biochem. 2004 Jul 1;330(1):52-7. DOI:10.1016/j.ab.2004.03.064 | PubMed ID:15183761 | HubMed [3]
  4. Fletcher MP, Diggle SP, Cámara M, and Williams P. Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules. Nat Protoc. 2007;2(5):1254-62. DOI:10.1038/nprot.2007.158 | PubMed ID:17546021 | HubMed [4]
  5. Noireaux V, Bar-Ziv R, and Libchaber A. Principles of cell-free genetic circuit assembly. Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12672-7. DOI:10.1073/pnas.2135496100 | PubMed ID:14559971 | HubMed [5]

All Medline abstracts: PubMed | HubMed

Purified Cell-Free Systems

  1. Cell-free translation systems for protein engineering by Yoshihiro Shimizu et al (2006)
  2. Efficient protein selection based on ribosome display system with purified components by Hiroyuki Ohashi et al (2006)

Pore Proteins for Cell-Free Exchange

  1. Rhee M and Burns MA. Nanopore sequencing technology: nanopore preparations. Trends Biotechnol. 2007 Apr;25(4):174-81. DOI:10.1016/j.tibtech.2007.02.008 | PubMed ID:17320228 | HubMed [1]
  2. Bakás L, Chanturiya A, Herlax V, and Zimmerberg J. Paradoxical lipid dependence of pores formed by the Escherichia coli alpha-hemolysin in planar phospholipid bilayer membranes. Biophys J. 2006 Nov 15;91(10):3748-55. DOI:10.1529/biophysj.106.090019 | PubMed ID:16935953 | HubMed [2]
  3. Sánchez-Magraner L, Cortajarena AL, Goñi FM, and Ostolaza H. Membrane insertion of Escherichia coli alpha-hemolysin is independent from membrane lysis. J Biol Chem. 2006 Mar 3;281(9):5461-7. DOI:10.1074/jbc.M512897200 | PubMed ID:16377616 | HubMed [3]
  4. Kerényi M, Allison HE, Bátai I, Sonnevend A, Emödy L, Plaveczky N, and Pál T. Occurrence of hlyA and sheA genes in extraintestinal Escherichia coli strains. J Clin Microbiol. 2005 Jun;43(6):2965-8. DOI:10.1128/JCM.43.6.2965-2968.2005 | PubMed ID:15956433 | HubMed [4]
  5. Subbarao GV and van den Berg B. Crystal structure of the monomeric porin OmpG. J Mol Biol. 2006 Jul 21;360(4):750-9. DOI:10.1016/j.jmb.2006.05.045 | PubMed ID:16797588 | HubMed [5]

All Medline abstracts: PubMed | HubMed