IGEM:Harvard/2006/DNA nanostructures/Logistics Communications

Communications

8-23

• See top of c5.0 Table of Working Stocks.

• Made up 1600uL of 300mM MgCl2 folding buffer from the HEPES pH 7.35 available in lab from Alain. In Shawn's box.

• Made up working stocks c5.0.E(b) and F(b), in c5.0 rack.

8-18

• Needs to be Ordered:
  • NEB 1kb ladder

• Needs to be Mixed Up:
  • Invitrogen 1kb+ ladder + loading dye solution (see below)

• Changes/Additions in Materials or their Positions:
  • Invitrogen 1kb+ ladder stock - in dark green rack (for 50mL -> 1.5mL tubes) on Bench 2
    • Invitrogen's manual says use 0.1ug/mm of lane
      • In the manual, 0.7ug (ie. 0.7uL) of ladder seems to look good...
  • c5.0.1
    • Mixed using 1-10uL multichannel pipettor
  • c5.0.4
  • c5.0.6
  • c5.0.7
    • NB: Next time, could whoever finishes the last of a pre-working stock PLEASE make some more, as a courtesy to others?
  • c5.0.Eb
  • c5.0.Fb
  • Proteinase K, reconstituted in 260uL dH2O, as seems to have been indicated by the bottle
    • Qiagen seems to have stopped producing this product in this lyophilized form - no instructions on this form were found online