Table of c5.0 Working Stocks

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Working Stock

NB: Noticed that c5.0A row included pre-working stocks 8 and 9, erroneously. These are biotinylated versions of 6 and 7, and should not have been included in previous stocks. Also, pre-working stocks 20 and 21 were erroneously included - these are displacement oligos, to be (putatively) added after latches are folded, with the lids present. Also, 10+11 is redundant with 6 and 7 - if latches are necessary, add 10+11; if no latches are desired, use 6 and 7. ---TC, 8.23.06

NB: Updated table to include lidless versions of c5.0C and c5.0D which have ligands pointing in and out respectively. ---KF, 11.14.06

Stock ID Experiment 1 2 3 4 4L 5 5L 6 7 8 9 10+11 12 13+14 15 16+17 18 19 20 21
c5.0A no latches, no aptamers x x x x - x - x - - - x x x x x x x - -
c5.0A (lidless) no latches, no aptamers 172uL - - 5 - 5 - 3 3 - - - - - - - - - - -
c5.0B latch design 2, no aptamers x - - x - x - - - - - x x x x x x - - -
c5.0C latch design 2, inside aptamers x x x x - - x - - - - x x x x x x - - -
c5.0.C (lidless) no latches, no lids, inside aptamers 172uL - - 5 - - 12 3 3 - - - - - - - - - - -
c5.0D latch design 2, outside aptamers, with lids x x x - x x - - - - - x x x x x x - - -
c5.0D (lidless) no latches, no lids, outside aptamers 172uL - - - 12 5 - 3 3 - - - - - - - - - - -
c5.0E(b) no latches, outside biotin 172uL - - - - 5 - 3 3 5 - - - - - - - - - -
c5.0F(b) no latches, intside biotin 172uL - - 5 - - - 3 3 - 5 - - - - - - - - -
c5.0E(b)r no latches, outside biotin 172uL - - - - 5 - 3 3 8 - - - - - - - - - -
c5.0F(b)r no latches, intside biotin 172uL - - 5 - - - 3 3 - 8 - - - - - - - - -

Pre-Working Stocks

Description Plate Locations Total
c5.0.1: core barrel oligos box_v5_A; box_v5_B rows A-F (1-12), row G (1-4) 172
c5.0.2: top lid oligos box_v5_C; box_v5_D row A (1-2) 98
c5.0.3: bottom lid oligos box_v5_D row A (3-12), rows B-H (1-12); box_v5_E row A (1-4) 98
c5.0.4: barrel oligos @ outside aptamers -aptamers box_v5_E row A (5-9) 5
c5.0.4L: barrel oligos @ outside aptamers+ligand box_v5_F row F (1-10) 10
c5.0.5: barrel oligos @ inside aptamers -aptamers box_v5_E row A (10-12), row B (1-2) 5
c5.0.5L: barrel oligos @ inside aptamers+ligand box_v5_F row F (11-12), row G (1-10) 12
c5.0.6: barrel oligos @ top latches -latches box_v5_E row B (3-5) 3
c5.0.7: barrel oligos @ bottom latches -latches box_v5_E row B (6-8) 3
c5.0.8: barrel oligos @ outside aptamers +biotin tubes c5.0.8.1-5 5
c5.0.9: barrel oligos @ inside aptamers +biotin tubes c5.0.9.1-5 5
c5.0.8r: revised barrel oligos @ outside aptamers +biotin tubes c5.0.8.1ra & b, c5.0.8.2ra & b, c5.0.8.5ra &b, plus c5.0.8.3 and c5.0.8.4 8
c5.0.9r: revised barrel oligos @ inside aptamers +biotin tubes c5.0.9.1ra & b - c5.0.9.5ra & b 8
c5.0.10+c5.0.11: barrel oligos @ latches (for latches) box_v5_F row A (1-9) 9
c5.0.12: barrel latch zippers box_v5_F row A (10-12), row B (1-3) 6
c5.0.13+c5.0.14: top lid latches box_v5_F row B (4-8) 5
c5.0.15: top lid latch zippers box_v5_F row B (9-11) 3
c5.0.16+c5.0.17:bottom lid latches box_v5_F row B (12), row C (1-5) 6
c5.0.18: bottom lid latch zippers box_v5_F row C (6-8) 3
c5.0.19: barrel displacement oligos box_v5_F row C (9-12), row D (1-2) 6
c5.0.20: top lid displacement oligos box_v5_F row D (3-5) 3
c5.0.21: bottom lid displacement oligos box_v5_F row D (6-8) 3

Totals

Working Stocks Description Pre-Working Stocks Water Total
c5.0A no latches, no aptamers, no lids 1 (86 μL), 4 (2.5 μL), 5 (2.5 μL), 6 (1.5 μL), 7 (1.5 μL) 106 μL 200 μL
c5.0A.lids no latches, no aptamers 2 (49 μL), 3 (49 μL) 102 μL 200 μL
c5.0C.core latch 2, in aptamers 1 (86 μL), 2 (49 μL), 3 (49 μL), 4 (2.5 μL), 5L (6 μL) - -
c5.0C.latches latch 2, in aptamers 10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL)) - -
c5.0D.core latch 2, out aptamers 1 (86 μL), 2 (49 μL), 3 (49 μL), 4L (5 μL), 5 (2.5 μL) - -
c5.0D.latches latch 2, out aptamers 10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL)) - -