IGEM:Cambridge/2008/Notebook/Voltage/2008/08/01

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Mutants The OD600 of the mutants grown in different K+ concentrations was taken again. osmY Plasmid mini prep was done. Gel was run, showing clear band at around 450bp, indicating osmY with BioBrick primer prefix and suffix. Glycerol stocks were made of the TOP10 cultures with the osmY-containing plasmid. More LB was added to the TOP10 cultures and they were put in the incubator. Plasmid PSB4C5 was extracted from DB31, into 100μl SDW - final conc. 106.5ng/μl.