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  • The OD600 of the mutants grown in different K+ concentrations was taken again.


  • Plasmid mini prep was done.
  • Gel was run, showing clear band at around 450bp, indicating osmY with BioBrick primer prefix and suffix.
  • Glycerol stocks were made of the TOP10 cultures with the osmY-containing plasmid.
  • More LB was added to the TOP10 cultures and they were put in the incubator.


  • PSB4C5 was extracted from DB31, into 100μl SDW - final conc. 106.5ng/μl.