IGEM:Cambridge/2008/Notebook/Voltage/2008/07/31

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Mutants

  • Mutant strains were grown in different potassium levels: 1mM, 50mM and 200mM.
  • Growth curves were plotted, using OD600 readings every 30 minutes for 3 hours.

Kdp

  • Ligated Kdp into death vector PSB4C5 again.
  • More PCR of gene from MG1655.

OsmY

  • TOP10 containing osmY-containing plasmid were grown in LB.