IGEM:Caltech/2007/Notes

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iGEM 2007

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Background

Initial assignment

Week 1

Notes

Week 2

Reading

  1. Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature. 2000 Jan 20;403(6767):335-8. DOI:10.1038/35002125 | PubMed ID:10659856 | HubMed [repressilator]
  2. Gardner TS, Cantor CR, and Collins JJ. Construction of a genetic toggle switch in Escherichia coli. Nature. 2000 Jan 20;403(6767):339-42. DOI:10.1038/35002131 | PubMed ID:10659857 | HubMed [toggle]
  3. [aisb]
All Medline abstracts: PubMed | HubMed

Notes

Week 3

Reading

  1. Fung E, Wong WW, Suen JK, Bulter T, Lee SG, and Liao JC. A synthetic gene-metabolic oscillator. Nature. 2005 May 5;435(7038):118-22. DOI:10.1038/nature03508 | PubMed ID:15875027 | HubMed [metabolator]
  2. Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, Sarpong R, and Keasling JD. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13;440(7086):940-3. DOI:10.1038/nature04640 | PubMed ID:16612385 | HubMed [artemisinic]
  3. Anderson JC, Clarke EJ, Arkin AP, and Voigt CA. Environmentally controlled invasion of cancer cells by engineered bacteria. J Mol Biol. 2006 Jan 27;355(4):619-27. DOI:10.1016/j.jmb.2005.10.076 | PubMed ID:16330045 | HubMed [tumor]
  4. You L, Cox RS 3rd, Weiss R, and Arnold FH. Programmed population control by cell-cell communication and regulated killing. Nature. 2004 Apr 22;428(6985):868-71. DOI:10.1038/nature02491 | PubMed ID:15064770 | HubMed [population]
All Medline abstracts: PubMed | HubMed

Notes

  • coming shortly

Week 4

Past Projects

Week 5

  • FimE [8] (Haile)
  • Crescentin [9] (Kat)
  • T7 [10] (Deborah)
  • SSM [11](Tania)
  • Riboregulator [12] (Pradeep)

Papers

  1. Ham TS, Lee SK, Keasling JD, and Arkin AP. A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Biotechnol Bioeng. 2006 May 5;94(1):1-4. DOI:10.1002/bit.20916 | PubMed ID:16534780 | HubMed [fim]
  2. Margolin W. Bacterial shape: concave coiled coils curve caulobacter. Curr Biol. 2004 Mar 23;14(6):R242-4. DOI:10.1016/j.cub.2004.02.057 | PubMed ID:15043836 | HubMed [shape]
  3. Chan LY, Kosuri S, and Endy D. Refactoring bacteriophage T7. Mol Syst Biol. 2005;1:2005.0018. DOI:10.1038/msb4100025 | PubMed ID:16729053 | HubMed [refactor]
  4. Torres-Cruz J and van der Woude MW. Slipped-strand mispairing can function as a phase variation mechanism in Escherichia coli. J Bacteriol. 2003 Dec;185(23):6990-4. DOI:10.1128/JB.185.23.6990-6994.2003 | PubMed ID:14617664 | HubMed [phase]
  5. Isaacs FJ, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, and Collins JJ. Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol. 2004 Jul;22(7):841-7. DOI:10.1038/nbt986 | PubMed ID:15208640 | HubMed [riboregulator]
All Medline abstracts: PubMed | HubMed

Week 6

  1. Isaacs FJ, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, and Collins JJ. Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol. 2004 Jul;22(7):841-7. DOI:10.1038/nbt986 | PubMed ID:15208640 | HubMed [riboregulator]
  2. Court DL, Oppenheim AB, and Adhya SL. A new look at bacteriophage lambda genetic networks. J Bacteriol. 2007 Jan;189(2):298-304. DOI:10.1128/JB.01215-06 | PubMed ID:17085553 | HubMed [lambda]
All Medline abstracts: PubMed | HubMed