IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/14

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8/14

Competent cells showed no growth, so new cells need to be prepared.

  • Started a seed of DH1b cells from Nielsen's lab.

Ethanol

Miniprepped PACYC plasmid

Prepared competent cells of BL21 strain to transform pacyc plasmid into.

  • Grew cells to O.D. of 3.1, followed procedure outlined by Nielsen Lab Procedures.