8/13
Competent Cell Check
- Ligation results suggest a problem with competent cells.
- Transformed 3 μL of TetR promoter into 3 tubes of 50 μL of comp. cells taken from 2 different tubes.
Ligation
- Ligation from 8/12 did not show any growth on any plates, suggesting competent cells may be bad.
- Started a new ligation to save until competent cells can be checked.
TesA+RBS insert with LacI+backbone
Ligation reaction
Reagent
|
Ligation (μL)
|
Control (μL)
|
TesA+RBS |
2.0 |
0.0
|
LacI |
2.0 |
2.0
|
Ligation Buffer |
1.0 |
1.0
|
Ligase |
1.0 |
1.0
|
H2O |
4.0 |
6.0
|
Ethanol
- Started seed of PACYC from plate started yesterday.
- Started seed of BL21 in preparation to make competent cells.
|