Haynes Lab:Notebook/Short Projects/2015/06/02
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Ben Nyer - 02/06/15
Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene
All 7 cultures showed growth in LB-Amp liquid medium. Performed plasmid minipreps and eluted in 50 µL DNase-free H2O.
Nucleic acid quantification:
Incubate at 37°C for 10 minutes, then run total volume on the gel. Expected size fragments are 1.2kb and 2.0kb.
shadow effect probably because the table was bumped during imaging.
Submit 200ng DNA + 1 µL of 10 µM primer (René's P139 primer works) in 10 µL total volume (make up difference with DNase-free H2O).