Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/15
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 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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Plasmid Maxiprep of KAH187-MV8--Notes-- During the plasmid purification procedure, I accidentally mixed the lysate flowthrough (containing plasmid) with the column preparation solution that was run through the Binding Column. Proceeding as normal from this point forward. Hopefully doesn't influence yield. --DNA Quantification-- Measured using the Take3 plate:
--Restriction Enzyme Digest--
Digest for 15 minutes at 37°C. --Gel Electrophoresis--
Expected size fragments from EcoRI/ApaLI digestion: 3951, 1243, 1135, 288, 234. Gel was run a little too long, ~300bp fragment sizes are difficult to visualize.
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