Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/10

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines

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Summary I'm submitting four RNA samples to DNASU today. They are: SKNSH: transfected with KAH126/MV2 SKNSH: negative control U2OS: transfected with KAH126/MV2 U2OS: negative control The SKNSH cells were transfected on 2/5 and harvested on 2/9. The U2OS cells were transfected on 1/22 and harvested on 1/27. Goals ✓ Quantify RNA in samples ✓ Submit samples to DNASU Protocol example protocol Notes Observations, measurements, placeholder info etc. go here. Results/Conclusions Plate reader data for RNA samples: SKNSH, KAH126/MV2: 548.4515 ng/μL SKNSH, neg control: 440.6735 ng/μL U2OS, KAH126/MV2: 662.9805 ng/μL U2OS, neg control: 728.3075 ng/μL Full data: Sample ID Replicate 1 Replicate 2 Average Reading SKNSH transfected w/ KAH126/MV2 0.69 0.681 0.6855 260 0.331 0.327 0.329 280 2.081 2.086 2.0835 260/280 551.82 545.083 548.4515 ng/µL SKNSH negative control 0.562 0.54 0.551 260 0.27 0.26 0.265 280 2.078 2.076 2.077 260/280 449.5 431.847 440.6735 ng/µL U2OS transfected w/ KAH126/MV2 0.862 0.795 0.8285 260 0.415 0.385 0.4 280 2.078 2.066 2.072 260/280 689.651 636.31 662.9805 ng/µL U2OS negative control 0.895 0.926 0.9105 260 0.426 0.443 0.4345 280 2.102 2.091 2.0965 260/280 715.765 740.85 728.3075 ng/µL