Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/09

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines

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Summary Following up on the transfection of SKNSH cells last Thursday, today I'm going to image them again and make a decision on whether to harvest for RNA-seq. Also need to start a culture of K-562 cells in preparation for transfecting them with PcTF-RFP. Goals ✓ Fluorescence microscopy of SKNSH cells transfected with PcTF-RFP ✓ If cells look good, harvest RNA using RNEasy method ✓ Thaw K562 cells, start culture Protocol TRIzol/RNeasy protocol Thawing cells Notes Observations, measurements, placeholder info etc. go here. Results/Conclusions Fluorescence microscopy images: Harvested the cells (2 biological replicates, 1 control), RNA is in the -80°C freezer. K562 culture is in PS-α, in the incubator. Potential plasmids to use as positive control in transfection/nucleofection: KAH133 (mCherry in MV2), KAH187 (CFP in MV8)