Haynes Lab:Notebook/Jan/2015/03/18

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Plasmid Transformations of MV2, BD004, BD006

  • Plasmid name: MV2
  • BioBrick name: None
  • Benchling link: MV2
  • Ampicillin resistance
  • Length of plasmid: 4529 bp
  • Transformation done on 3/17/2015
  • Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success.
  • Result for Plate 2: No growth anywhere. Success.


  • Plasmid name: BD004
  • BioBrick name: None (composed of DPRE-5xGal4-spacer-HPK-Kozak-AmCyan-AmCyan-NLS-Stop-PolyA)
  • Benchling link: BD004
  • Ampicillin resistance
  • Length of plasmid: 4116 bp
    • Correction: Plasmid length is insert 4116 + V0120 vector 3200 = 7316 bp
  • Transformation done on 3/17/2015
  • Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success.
  • Result for Plate 2: No growth anywhere. Success.


  • Plasmid name: BD006
  • BIobrick name: None (composed of DPRE-5xGal4-spacer-CMV-Kozak-AmCyan-AmCyan-NLS-Stop-PolyA)
  • Benchling link: BD006
  • Ampicillin resistance
  • Length of plasmid: 4182 bp
    • Correction: Plasmid length is insert 4182 + V0120 vector 3200 = 7382 bp
  • Transformation done on 3/17/2015
  • Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success.
  • Result for Plate 2: No growth anywhere. Success.