Haynes Lab:Notebook/Jan/2015/03/19

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03/19/15

  • Plasmid preps - overnight cultures (Dr. Haynes)



Plasmid preps - overnight cultures

5 mL liquid cultures, pick 2 colonies from each of Jan's plates

  1. MV2 colony a
  2. MV2 colony b
  3. BD004 colony a
  4. BD004 colony b
  5. BD006 colony a
  6. BD006 colony b
  • Wear fresh gloves
  • Use sterile pipette tip to pick colony, streak onto fresh agar, drop tip into liquid medium
  • Grow streak plate and cultures overnight at 37°C; liquid cultures grown with shaking