Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/02/09
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DBN011 ligation results:
Going to pick 4 colonies from the treatment plate and proceed with streaks & liquid cultures.
Incubate at 37 °C for 10 minutes, then gel purify. HA1 part is 106 bp, DBN011 part is 5769 bp.
Gel Extraction Results
HA1 part looks good, but very faint band. DBN011 1, 2, and 4 look good. Proceeding to extract 1 and 2.
Incubate at room temperature for 10 minutes, then take 1 µL of reaction volume and use for quick transformation. Count colonies tomorrow.