Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/02/08
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More adventures in cloning...
quantification went fine, concentrations are between 50 and 70 ng/µL for DBN010. A bit lower for HA1_v0120, between 7 and 24 ng/µL. 260/280 values are good.
Incubate at 37 °C for 10 minutes, then purify via gel electrophoresis.
All four plasmids appear to contain the insert. Will proceed with gel extraction from colonies 1 and 3.
DNA Quantification, post-purification
Concentration is ~7 ng/µL for both samples. 260/280 is on the high side, around 2.8-2.9. Going to proceed with ligation...
Incubate at room temperature for 10 minutes, then take 1 µL from each sample (treatment & negative control) and use for quick transformation protocol.