Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/08/07

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Overview

Cleaned samples using the Qiaquick PCR Purification kit. Post-cleanup sample concentration:

average (ng/µL) st dev (ng/µL) 260/280
9.4295 2.540634665 2.6725
23.9095 0.420728535 1.948

Gel image:

File:2015-08-07 Gal4EED-luc PCR products.TIF

Both bands are the right approximate size. Sending four samples to DNASU for sequencing (2 amplicons * 2 primers per amplicon)