Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/08/05
|Sequence verification around tk-luc (cont'd)||Main project page|
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Trying to repeat the PCR for the first reaction using P197 & P215 primers (René's).
Reaction 1 (anneal temp. 54°C):
Gel Electrophoresis Results
Faint but visible bands at all temperature ranges (46-->54). Must have not put in one of the primers during the previous run. Will perform PCR clean-up and submit samples for sequencing in a couple days.