Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/07/15
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All of the cultures that were expanded into T-25 flasks look mostly dead, with little cell adhesion save for a few scattered clumps. I may have to repeat with less aggressive expansion next time. Will keep an eye on the cultures regardless, and analyzing via flow cytometry when sufficient density is reached.