Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/25

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

03/25/2015

PCR Purification of a double digest reaction of my ligation created 3-19-2015. I used EcoR1 and XBaI
This will be the "new backbone" used to insert synthase genes in the future.

Sample Read# 260 280 260/280 ng/µL
1 0.01 0.007 1.516 9.897


I PCR-purified to get rid of the insert because Ryan designed the insert to be small enough that it will be washed away during the purification. As a result, our product should consist of just the backbone.